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231223s2010 xx |||||o 00| ||eng c |
| 024 |
7 |
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|a 10.1016/j.clim.2010.09.002
|2 doi
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2 |
|a pubmed24n0674.xml
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|a (NLM)20920845
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Achenbach, Peter
|e verfasserin
|4 aut
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| 245 |
1 |
2 |
|a A simplified method to assess affinity of insulin autoantibodies
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| 264 |
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1 |
|c 2010
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 26.11.2010
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| 500 |
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|a Date Revised 08.11.2010
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a Copyright © 2010 Elsevier Inc. All rights reserved.
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| 520 |
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|a Insulin autoantibodies (IAA) precede type 1 diabetes, but not all IAA-positive children develop other islet autoantibodies and disease. Diabetes risk can be stratified by laborious IAA affinity measurement using competition with multiple ligand concentrations. Here, we identify a single competitor concentration that discriminates low- and high-affinity IAA. Discrimination was achieved among 122 IAA-positive sera using 7.0 nM competitor which is 54-fold that of the assay radioligand concentration. Relative-binding <60% at this competitor concentration identified all 85 sera with affinities ≥1.0×10⁸ L/mol and none with lower affinities (P<0.0001), and 45 (96%) of 47 multiple islet autoantibody-positive sera (P<0.0001). IAA competition was further tested in a second set of 119 IAA-positive sera. Of these, 99 fulfilled high-affinity competition criteria of <60% relative-binding at 7.0 nM competitor including 89 (94%) of 95 sera with multiple islet autoantibodies (P<0.0001). Thus, increased IAA specificity can be achieved with simple modification to existing assays
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| 650 |
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4 |
|a Journal Article
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| 650 |
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4 |
|a Research Support, Non-U.S. Gov't
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| 650 |
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7 |
|a Autoantibodies
|2 NLM
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| 650 |
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7 |
|a Insulin Antibodies
|2 NLM
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| 700 |
1 |
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|a Guo, Liang-Hao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gick, Claudia
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Adler, Kerstin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Krause, Stephanie
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bonifacio, Ezio
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Colman, Peter G
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ziegler, Anette-G
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Clinical immunology (Orlando, Fla.)
|d 1999
|g 137(2010), 3 vom: 15. Dez., Seite 415-21
|w (DE-627)NLM098196855
|x 1521-7035
|7 nnns
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| 773 |
1 |
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|g volume:137
|g year:2010
|g number:3
|g day:15
|g month:12
|g pages:415-21
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| 856 |
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0 |
|u http://dx.doi.org/10.1016/j.clim.2010.09.002
|3 Volltext
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