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231223s2010 xx |||||o 00| ||eng c |
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|a 10.2166/wst.2010.818
|2 doi
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|a DE-627
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Mayer, B K
|e verfasserin
|4 aut
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| 245 |
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|a Development and validation of an integrated cell culture-qRTPCR assay for simultaneous quantification of coxsackieviruses, echoviruses, and polioviruses in disinfection studies
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|c 2010
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 30.03.2010
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|a Date Revised 10.12.2019
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|a published: Print
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|a Citation Status MEDLINE
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|a This study demonstrated the applicability of integrated cell culture-quantitative RTPCR (ICC-qRTPCR) for the simultaneous quantification of coxsackievirus, echovirus, and poliovirus in disinfection studies. Buffalo green monkey cells were inoculated with a 10-fold dilution series of mixed enteroviruses and incubated prior to qRTPCR quantification. Optimal assay conditions included three post infection washes and a 24-hour post infection incubation period based on successful differentiation between infectious and noninfectious viruses and significant and consistent viral replication rates. Ultraviolet disinfection studies were performed to validate the ICC-qRTPCR assay. Using the optimized assay, three-log microbial inactivation was achieved at UV doses of 30-44, 28-42, and 28-29 mJ/cm(2) for coxsackievirus B6, echovirus 12, and poliovirus 1, respectively. These results compare favorably to side-by-side assessments using conventional cultural techniques and values previously reported in the literature. This indicates that ICC-qRTPCR is a practical alternative for the simultaneous quantification of enteroviruses in disinfection studies
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|a Journal Article
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|a Research Support, U.S. Gov't, Non-P.H.S.
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| 700 |
1 |
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|a Ryu, H
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gerrity, D
|e verfasserin
|4 aut
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| 700 |
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|a Abbaszadegan, M
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Water science and technology : a journal of the International Association on Water Pollution Research
|d 1986
|g 61(2010), 2 vom: 21., Seite 375-87
|w (DE-627)NLM098149431
|x 0273-1223
|7 nnas
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| 773 |
1 |
8 |
|g volume:61
|g year:2010
|g number:2
|g day:21
|g pages:375-87
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| 856 |
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|u http://dx.doi.org/10.2166/wst.2010.818
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|d 61
|j 2010
|e 2
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|h 375-87
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