A quantitative approach to detect and overcome PCR inhibition in ancient DNA extracts

Inhibition is problematic in many applications of PCR, particularly those involving degraded or low amounts of template DNA, when simply diluting the extract is undesirable. Two basic approaches to monitoring inhibition in such samples using real-time or quantitative PCR (qPCR) have been proposed. T...

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Veröffentlicht in:BioTechniques. - 1993. - 47(2009), 5 vom: 01. Nov., Seite 941-9
1. Verfasser: King, Christine (VerfasserIn)
Weitere Verfasser: Debruyne, Régis, Kuch, Melanie, Schwarz, Carsten, Poinar, Hendrik
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2009
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't DNA 9007-49-2