A quantitative approach to detect and overcome PCR inhibition in ancient DNA extracts
Inhibition is problematic in many applications of PCR, particularly those involving degraded or low amounts of template DNA, when simply diluting the extract is undesirable. Two basic approaches to monitoring inhibition in such samples using real-time or quantitative PCR (qPCR) have been proposed. T...
Ausführliche Beschreibung
Bibliographische Detailangaben
Veröffentlicht in: | BioTechniques. - 1993. - 47(2009), 5 vom: 01. Nov., Seite 941-9
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1. Verfasser: |
King, Christine
(VerfasserIn) |
Weitere Verfasser: |
Debruyne, Régis,
Kuch, Melanie,
Schwarz, Carsten,
Poinar, Hendrik |
Format: | Online-Aufsatz
|
Sprache: | English |
Veröffentlicht: |
2009
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Zugriff auf das übergeordnete Werk: | BioTechniques
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Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
DNA
9007-49-2 |