In vitro cell-toxicity screening as an alternative animal model for coral toxicology : effects of heat stress, sulfide, rotenone, cyanide, and cuprous oxide on cell viability and mitochondrial function
The logistics involved in obtaining and maintaining large numbers of corals hampers research on the toxicological effects of environmental contaminants for this ecologically and economically important taxon. A method for creating and culturing single-cell suspensions of viable coral cells was develo...
| Veröffentlicht in: | Ecotoxicology (London, England). - 1992. - 19(2010), 1 vom: 12. Jan., Seite 171-84 |
|---|---|
| 1. Verfasser: | |
| Weitere Verfasser: | , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2010
|
| Zugriff auf das übergeordnete Werk: | Ecotoxicology (London, England) |
| Schlagworte: | Journal Article Environmental Pollutants Fluorescent Dyes Formazans Sulfides Tetrazolium Salts Rotenone 03L9OT429T MTT formazan 23305-68-2 mehr... |
| Zusammenfassung: | The logistics involved in obtaining and maintaining large numbers of corals hampers research on the toxicological effects of environmental contaminants for this ecologically and economically important taxon. A method for creating and culturing single-cell suspensions of viable coral cells was developed. Cell segregation/separation was based on specific cell densities and resulting cell cultures were viable for at least 2 mos. Low-density cells lacking symbiotic zooxanthallae and rich in mitochondria were isolated and cultured for toxicity studies. Cells were exposed to differing degrees or concentrations of heat stress, rotenone, cyanide, sulfide, and cuprous oxide. Cells were assayed for mitochondrial membrane potential using the fluorescent probe, JC-9, and for overall viability using the MTT/formazan spectrophotometric viability assay. Significant differences were observed between controls and treatments and the efficacy of this method was validated; only 2 cm(2) of tissue was required for a seven-point concentration-exposure series |
|---|---|
| Beschreibung: | Date Completed 22.03.2010 Date Revised 20.10.2021 published: Print Citation Status MEDLINE |
| ISSN: | 1573-3017 |
| DOI: | 10.1007/s10646-009-0403-5 |