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231223s2009 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erp153
|2 doi
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|a pubmed24n1222.xml
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|a (NLM)19454595
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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1 |
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|a Johansen, Jorunn Nergaard
|e verfasserin
|4 aut
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| 245 |
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|a AtRAB-H1b and AtRAB-H1c GTPases, homologues of the yeast Ypt6, target reporter proteins to the Golgi when expressed in Nicotiana tabacum and Arabidopsis thaliana
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|c 2009
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 19.10.2009
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|a Date Revised 13.12.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Ypt/Rab GTPases act as key regulators of intracellular traffic through the conformational differences exhibited by their GTP or GDP-bound forms. In this paper, two Arabidopsis Ypt6 homologues, AtRAB-H1(b) and AtRAB-H1(c) were characterized and compared. Using a live cell imaging approach, it is shown that yellow fluorescent protein-fusions (YFP) of AtRAB-H1(b) and AtRAB-H1(c) locate to the Golgi and to the cytosol in both Nicotiana tabacum and in Arabidopsis thaliana. In addition, YFP-AtRAB-H1(b) targets an as yet unknown compartment not labelled by YFP-AtRAB-H1(c) or Golgi markers. It is also shown that the subcellular location of YFP-AtRAB-H1(b) and YFP-AtRAB-H1(c) is affected by the state of GTP-binding and that expression of a GTP-deficient mutant results in increased apoplastic fluorescence of a secretory form of YFP
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Arabidopsis Proteins
|2 NLM
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|a Recombinant Fusion Proteins
|2 NLM
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|a Guanosine Triphosphate
|2 NLM
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|a 86-01-1
|2 NLM
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| 650 |
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|a Rab-H1b protein, Arabidopsis
|2 NLM
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| 650 |
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|a EC 3.6.1.-
|2 NLM
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| 650 |
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|a Rab-H1c protein, Arabidopsis
|2 NLM
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| 650 |
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|a EC 3.6.1.-
|2 NLM
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| 650 |
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|a rab GTP-Binding Proteins
|2 NLM
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| 650 |
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|a EC 3.6.5.2
|2 NLM
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| 700 |
1 |
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|a Chow, Cheung-Ming
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Moore, Ian
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hawes, Chris
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 60(2009), 11 vom: 08., Seite 3179-93
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:60
|g year:2009
|g number:11
|g day:08
|g pages:3179-93
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|u http://dx.doi.org/10.1093/jxb/erp153
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