Ultrastructural Cytochemistry of Secretory Granules of Esophageal Glands of Meloidogyne incognita

Ultrastructural cytochemical tests for several enzymes, proteins, carbohydrates, and nucleic acids were conducted on secretory granules o pound dorsal and subventral esophageal glands of preparasitic second-stage juveniles and the dorsal gland of adult females of Meloidogyne incognita. Secretory gra...

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Veröffentlicht in:Journal of nematology. - 1969. - 20(1988), 1 vom: 15. Jan., Seite 141-9
1. Verfasser: Sundermann, C A (VerfasserIn)
Weitere Verfasser: Hussey, R S
Format: Aufsatz
Sprache:English
Veröffentlicht: 1988
Zugriff auf das übergeordnete Werk:Journal of nematology
Schlagworte:Journal Article Meloidogyne incognita cytochemistry electron microscopy enzyme esophageal gland root-knot nematode secretory granule
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245 1 0 |a Ultrastructural Cytochemistry of Secretory Granules of Esophageal Glands of Meloidogyne incognita 
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520 |a Ultrastructural cytochemical tests for several enzymes, proteins, carbohydrates, and nucleic acids were conducted on secretory granules o pound dorsal and subventral esophageal glands of preparasitic second-stage juveniles and the dorsal gland of adult females of Meloidogyne incognita. Secretory granules in the subventral glands of juveniles stained positive for acid phosphatase. Peroxidase, DNase, RNase, cellulase, and nucleic acids were not detected in these granules. Secretory granules in the dorsal gland of adult females stained positive for peroxidase (pH 7.6) in < 50% of the tests, Acid phosphatase, beta-glucuronidase, DNase, RNase, polyphenoloxidase, cellulase, and carbohydrates were not detected in dorsal gland granules in adult females. Positive staining with cobalt thiocyanate, a stain for amino groups of basic proteins, occurred in secretory granules in the dorsal gland, ribosomes, and chromatin in adult females. Ribosomes, nuclei, and secretory granules of the dorsal gland of adult females intensely stained when incubated in three reagents specific for nucleic acid 
650 4 |a Journal Article 
650 4 |a Meloidogyne incognita 
650 4 |a cytochemistry 
650 4 |a electron microscopy 
650 4 |a enzyme 
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650 4 |a root-knot nematode 
650 4 |a secretory granule 
700 1 |a Hussey, R S  |e verfasserin  |4 aut 
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