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NLM187021961 |
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DE-627 |
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20250210061918.0 |
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tu |
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231223s1993 xx ||||| 00| ||eng c |
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2 |
|a pubmed25n0623.xml
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|a (DE-627)NLM187021961
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|a (NLM)19279761
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Wergin, W P
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Use of low temperature scanning electron microscopy to observe frozen hydrated specimens of nematodes
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| 264 |
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1 |
|c 1993
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 14.07.2011
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|a Date Revised 20.10.2021
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|a published: Print
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|a Citation Status PubMed-not-MEDLINE
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|a Frozen hydrated specimens of Pratylenchus agilis and dauer larvae of Steinernema carpocapsae were observed with low-temperature field emission scanning electron microscopy. This new technique provides information about the surface features of nematodes and also allows specimens to be fractured to reveal their internal structure. Furthermore, both halves of fractured specimens can be retained, examined, and photographed either as two-dimensional micrographs or as three-dimensional images for stereo observation (stereology) or quantitative measurements (stereometry). This technique avoids artifacts normally associated with procedures required to prepare nematodes for examination in the transmission and scanning electron microscopes, such as chemical fixation, dehydration, and sectioning or critical point drying
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| 650 |
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|a Journal Article
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| 650 |
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|a Complementary imaging
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| 650 |
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4 |
|a Pratylenchus agilis
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| 650 |
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4 |
|a SEM
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| 650 |
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4 |
|a Steinernema carpocapsae
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| 650 |
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4 |
|a low-temperature SEM
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| 650 |
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4 |
|a nematode
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| 650 |
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|a stereology
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| 650 |
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4 |
|a technique
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| 650 |
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4 |
|a three-dimensional imaging
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| 700 |
1 |
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|a Sayre, R M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Erbe, E F
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of nematology
|d 1969
|g 25(1993), 2 vom: 10. Juni, Seite 214-26
|w (DE-627)NLM098196421
|x 0022-300X
|7 nnns
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| 773 |
1 |
8 |
|g volume:25
|g year:1993
|g number:2
|g day:10
|g month:06
|g pages:214-26
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| 912 |
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 25
|j 1993
|e 2
|b 10
|c 06
|h 214-26
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