In-situ Hybridization to Messenger RNA in Heterodera glycines

A method is presented for in-situ hybridization to mRNA in second-stage juveniles (J2) of the soybean cyst nematode Heterodera glycines. The protocol was developed using a digoxigenin-labeled RNA probe transcribed from cDNA of a cellulase gene that was known to be expressed in the subventral esophag...

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Détails bibliographiques
Publié dans:Journal of nematology. - 1969. - 30(1998), 3 vom: 09. Sept., Seite 309-12
Auteur principal: de Boer, J M (Auteur)
Autres auteurs: Yan, Y, Smant, G, Davis, E L, Baum, T J
Format: Article
Langue:English
Publié: 1998
Accès à la collection:Journal of nematology
Sujets:Journal Article Heterodera glycines cellulase gene digoxigenin RNA probe esophageal gland in-situ hybridization nematode