A Method for Isolation of Pasteuria penetrans Endospores for Bioassay and Genomic Studies
A rapid method for collection of Pasteuria penetrans endospores was developed. Roots containing P. penetrans-infected root-knot nematode females were softened by pectinase digestion, mechanically processed, and filtered to collect large numbers of viable endospores. This method obviates laborious ha...
| Publié dans: | Journal of nematology. - 1969. - 38(2006), 1 vom: 28. März, Seite 165-7 |
|---|---|
| Auteur principal: | |
| Autres auteurs: | , |
| Format: | Article |
| Langue: | English |
| Publié: |
2006
|
| Accès à la collection: | Journal of nematology |
| Sujets: | Journal Article |
| Résumé: | A rapid method for collection of Pasteuria penetrans endospores was developed. Roots containing P. penetrans-infected root-knot nematode females were softened by pectinase digestion, mechanically processed, and filtered to collect large numbers of viable endospores. This method obviates laborious handpicking of Pasteuria-infected females and yields endospores competent to attach to and infect nematodes. Endospores are suitable for morphology studies and DNA preparations |
|---|---|
| Description: | Date Completed 25.09.2012 Date Revised 20.10.2021 published: Print Citation Status PubMed-not-MEDLINE |
| ISSN: | 0022-300X |