Adsorption of calf thymus DNA on Au(110) studied by reflection anisotropy spectroscopy
Reflection anisotropy spectroscopy (RAS) was used to investigate the adsorption of single-stranded (ss-) and double-stranded (ds-) calf thymus DNA on Au(110) in an electrochemical cell. Both types of DNA form ordered structures for electrode potentials in the range from +0.6 to -0.4 V. Both types of...
| Publié dans: | Langmuir : the ACS journal of surfaces and colloids. - 1985. - 23(2007), 4 vom: 13. Feb., Seite 2078-82 |
|---|---|
| Auteur principal: | |
| Autres auteurs: | , , , |
| Format: | Article |
| Langue: | English |
| Publié: |
2007
|
| Accès à la collection: | Langmuir : the ACS journal of surfaces and colloids |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't Gold 7440-57-5 DNA 9007-49-2 calf thymus DNA 91080-16-9 |
| Résumé: | Reflection anisotropy spectroscopy (RAS) was used to investigate the adsorption of single-stranded (ss-) and double-stranded (ds-) calf thymus DNA on Au(110) in an electrochemical cell. Both types of DNA form ordered structures for electrode potentials in the range from +0.6 to -0.4 V. Both types of DNA desorb at -0.6 V and may start desorbing at lower negative potentials. When adsorbed at +0.6 V, both forms give rise to a similar RAS signal and adsorb through the phosphate groups. As the potential is reduced, the RAS intensity observed from ss-DNA increases to roughly twice that observed from ds-DNA, a result that is interpreted as due to a change in the adsorption of the ss-DNA from sites involving the phosphate groups to sites involving the bases |
|---|---|
| Description: | Date Completed 02.05.2007 Date Revised 29.01.2022 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1520-5827 |