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231223s2006 xx ||||| 00| ||eng c |
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|a pubmed25n0556.xml
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|a (DE-627)NLM166814989
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|a (NLM)17129062
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Lai, Rebecca Y
|e verfasserin
|4 aut
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| 245 |
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|a Comparison of the signaling and stability of electrochemical DNA sensors fabricated from 6- or 11-carbon self-assembled monolayers
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|c 2006
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 19.01.2007
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|a Date Revised 03.12.2007
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|a published: Print
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|a Citation Status MEDLINE
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|a We have characterized the solution-phase and dry storage stability of electrochemical E-DNA sensors fabricated using mixed self-assembled monolayers (SAMs) composed of 6- or 11-carbon (C6 and C11, respectively) alpha,omega-thiol alcohols and the analogous C6- or C11-thiol-terminated stem-loop DNA probe. We find that the solution-phase and dry storage stability of C6-based E-DNA sensors are limited and poorly reproducible. The use of stabilizing agents bovine serum albumin plus either glucose or trehalose significantly improves the dry storage shelf life of such sensors: when using these preservatives, we observe only 7-9% sensor degradation after 1 month of storage in air at room temperature. In comparison, the stability of C11-based E-DNA sensors is significantly greater than that of the C6-based sensors; we observe only minor (5-8%) loss of signal upon storing these sensors for a week under ambient solution conditions or for more than a month in air in the presence of preservatives. Moreover, whereas the electron-transfer rate through C11 SAMs is slower than that observed for C6 SAMs, it is rapid enough to support good sensor performance. It thus appears that C11 SAMs provide a reasonable compromise between electron-transfer efficiency and sensor stability and are well suited for use in electronic DNA-sensing applications
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|a Comparative Study
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|a Journal Article
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|a Research Support, N.I.H., Extramural
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|a Research Support, U.S. Gov't, Non-P.H.S.
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|a Alcohols
|2 NLM
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|a Membranes, Artificial
|2 NLM
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|a Oligonucleotides
|2 NLM
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|a Sulfhydryl Compounds
|2 NLM
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|a Carbon
|2 NLM
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|a 7440-44-0
|2 NLM
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|a Gold
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|a 7440-57-5
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|a DNA
|2 NLM
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|a 9007-49-2
|2 NLM
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| 700 |
1 |
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|a Seferos, Dwight S
|e verfasserin
|4 aut
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|a Heeger, Alan J
|e verfasserin
|4 aut
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|a Bazan, Guillermo C
|e verfasserin
|4 aut
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| 700 |
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|a Plaxco, Kevin W
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1985
|g 22(2006), 25 vom: 05. Dez., Seite 10796-800
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnas
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| 773 |
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|g volume:22
|g year:2006
|g number:25
|g day:05
|g month:12
|g pages:10796-800
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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|a GBV_NLM
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|a GBV_ILN_721
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|a AR
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|d 22
|j 2006
|e 25
|b 05
|c 12
|h 10796-800
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