Two-step cross-linking method for identification of NF-kappaB gene network by chromatin immunoprecipitation

Two-step cross-linking method for identification of NF-kappaB gene network by chromatin immunoprecipitation

The chromatin immunoprecipitation (ChIP) assay has recently been exploited as a powerful and versatile technique for probing protein-DNA interactions within the chromatin environment. In this method, intact cells are fixed with a reversible DNA-protein cross-linking agent (formaldehyde), and associa...

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Veröffentlicht in:BioTechniques. - 1991. - 39(2005), 5 vom: 07. Nov., Seite 715-25
1. Verfasser: Nowak, David E (VerfasserIn)
Weitere Verfasser: Tian, Bing, Brasier, Allan R
Format: Aufsatz
Sprache:English
Veröffentlicht: 2005
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Chromatin Cross-Linking Reagents DNA Primers I-kappa B Proteins NF-kappa B NFKBIA protein, human RELA protein, human mehr... STAT3 Transcription Factor Succinimides Transcription Factor RelA NF-KappaB Inhibitor alpha 139874-52-5 DNA 9007-49-2 N-hydroxysuccinimide MJE3791M4T