Antigen binding forces of single antilysozyme Fv fragments explored by atomic force microscopy

Antigen binding forces of single antilysozyme Fv fragments explored by atomic force microscopy

We used atomic force microscopy (AFM) to explore the antigen binding forces of individual Fv fragments of antilysozyme antibodies (Fv). To detect single molecular recognition events, genetically engineered histidine-tagged Fv fragments were coupled onto AFM tips modified with mixed self-assembled mo...

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Détails bibliographiques
Publié dans:Langmuir : the ACS journal of surfaces and colloids. - 1985. - 21(2005), 12 vom: 07. Juni, Seite 5517-23
Auteur principal: Berquand, Alexandre (Auteur)
Autres auteurs: Xia, Nan, Castner, David G, Clare, Brian H, Abbott, Nicholas L, Dupres, Vincent, Adriaensen, Yasmine, Dufrêne, Yves F
Format: Article
Langue:English
Publié: 2005
Accès à la collection:Langmuir : the ACS journal of surfaces and colloids
Sujets:Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Antigen-Antibody Complex Immunoglobulin Fragments immunoglobulin Fv Muramidase EC 3.2.1.17