A prediction method for the isoelectric point of binary protein mixtures of bovine serum albumin and lysozyme adsorbed on colloidal titania and alumina particles
Bovine serum albumin and lysozyme mixtures of different mole fractions were adsorbed to colloidal alumina (116 nm) and titania particle (271 nm) suspensions of 2 vol % solid content for 16 h at pH 7.5. The total protein amount normalized to the powder surface area was 1000 ng/cm2. The zeta potential...
| Publié dans: | Langmuir : the ACS journal of surfaces and colloids. - 1991. - 21(2005), 8 vom: 12. Apr., Seite 3493-7 |
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| Auteur principal: | |
| Autres auteurs: | , |
| Format: | Article |
| Langue: | English |
| Publié: |
2005
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| Accès à la collection: | Langmuir : the ACS journal of surfaces and colloids |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't Colloids Water 059QF0KO0R titanium dioxide 15FIX9V2JP Serum Albumin, Bovine 27432CM55Q Titanium plus... |
| Résumé: | Bovine serum albumin and lysozyme mixtures of different mole fractions were adsorbed to colloidal alumina (116 nm) and titania particle (271 nm) suspensions of 2 vol % solid content for 16 h at pH 7.5. The total protein amount normalized to the powder surface area was 1000 ng/cm2. The zeta potential of the protein-treated suspensions was measured as a function of pH and the isoelectric point (IEP) obtained. A simple prediction model in two refinement steps was derived and evaluated for the obtained IEPs. The best model fit which takes into account moles of protein and surface fractions yielded an average prediction error of 7.5% and a maximum error of 16.7% |
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| Description: | Date Completed 08.08.2006 Date Revised 16.11.2017 published: Print Citation Status MEDLINE |
| ISSN: | 0743-7463 |