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NLM152978046 |
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DE-627 |
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231223s2005 xx ||||| 00| ||chi c |
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|a pubmed25n0510.xml
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|a (DE-627)NLM152978046
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|a (NLM)15636703
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a chi
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| 100 |
1 |
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|a Yang, Guang-ming
|e verfasserin
|4 aut
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1 |
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|a Effect of MCI-154 on calcium sensitivity of vascular smooth muscle and its mechanism in hemorrhagic shock in rats
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|c 2005
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 08.10.2009
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|a Date Revised 20.11.2014
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|a published: Print
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|a Citation Status MEDLINE
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|a OBJECTIVE: To observe the effects of new calcium sensitizer MCI-154 on calcium sensitivity of vascular smooth muscle following hemorrhagic shock (HS) in rats, and to explore its mechanism
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|a METHODS: The superior mesenteric artery (SMA) from HS model of rat was adopted to assay the calcium sensitivity via observing the contraction initiated by Ca2+ under depolarizing conditions (120 mmol/L K+) with isolated organ perfusion system. The experiment was conducted in two parts, the effects of MCI-154 on calcium sensitivity of vascular smooth muscle in HS, and to explore whether MCI-154 could regulate the calcium sensitivity through regulating Rho-kinase, protein kinase C (PKC) and protein kinase G(PKG)
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|a RESULTS: Compared with the normal control group, the cumulative dose-response curve of SMA to Ca2+ in shock group was shifted to the right, the maximal contraction to Ca2+ was decreased significantly (P<0.01). MCI-154(10(-7), 10(-6), 10(-5), 10(-4) mol/L) pretreatment further shift the cumulative dose-response curve of Ca2+ to the right as compared to shock group (P<0.01). These result suggested that calcium desensitization existed in the vascular smooth muscle following HS, and MCI-154 further decreased the calcium sensitivity. Angiotensin II and phorbol 12-myristate-13-acetate (PMA), the Rho-kinase agonist and PKC agonist, increased the calcium sensitivity, made the cumulative dose-response curve of Ca2+ shift to the left. After MCI-154(10(-5) mol/L) pretreatment, the dose-response curve of Ca2+ was shifted to the right as compared to Ang II and PMA alone group (P<0.01). But, KT-5823, the PKG antagonist, antagonized MCI-154-induced right shift of the dose-response curve of Ca2+. It was suggested that MCI-154 induced the decrease of calcium sensitivity might be related to Rho-kinase, PKC and PKG
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|a CONCLUSION: The calcium sensitivity of vascular smooth muscle following HS is significantly decreased, MCI-154 can further decrease calcium sensitivity. MCI-154 may regulate calcium sensitivity of vascular smooth muscle through Rho-kinase, PKC and PKG
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|a English Abstract
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Pyridazines
|2 NLM
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|a Vasodilator Agents
|2 NLM
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| 650 |
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|a MCI 154
|2 NLM
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| 650 |
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|a 98326-33-1
|2 NLM
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|a rho-Associated Kinases
|2 NLM
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| 650 |
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|a EC 2.7.11.1
|2 NLM
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|a Cyclic GMP-Dependent Protein Kinases
|2 NLM
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| 650 |
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|a EC 2.7.11.12
|2 NLM
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|a Protein Kinase C
|2 NLM
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| 650 |
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|a EC 2.7.11.13
|2 NLM
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| 650 |
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|a Calcium
|2 NLM
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| 650 |
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|a SY7Q814VUP
|2 NLM
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| 700 |
1 |
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|a Liu, Liang-ming
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue
|d 1998
|g 17(2005), 1 vom: 06. Jan., Seite 7-11
|w (DE-627)NLM098227793
|x 1003-0603
|7 nnns
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| 773 |
1 |
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|g volume:17
|g year:2005
|g number:1
|g day:06
|g month:01
|g pages:7-11
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|a AR
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|d 17
|j 2005
|e 1
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|c 01
|h 7-11
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