Determination of Na+ binding parameters by relaxation analysis of selected 23Na NMR coherences : RNA, BSA and SDS
Copyright 2004 John Wiley & Sons, Ltd.
| Veröffentlicht in: | Magnetic resonance in chemistry : MRC. - 1985. - 43(2005), 3 vom: 01. März, Seite 217-24 |
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| Weitere Verfasser: | , , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2005
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| Zugriff auf das übergeordnete Werk: | Magnetic resonance in chemistry : MRC |
| Schlagworte: | Comparative Study Journal Article Research Support, Non-U.S. Gov't Macromolecular Substances Micelles Serum Albumin, Bovine 27432CM55Q Sodium Dodecyl Sulfate 368GB5141J RNA mehr... |
| Zusammenfassung: | Copyright 2004 John Wiley & Sons, Ltd. Nuclear magnetic resonance provides several unique means of investigating the interactions between different inorganic ions and various macromolecules. (23)Na is a quadrupolar nucleus, meaning that relaxation analysis of the various coherences allows the measurement of its binding to biological macromolecules. In this study, we analyzed the quadrupolar relaxation of (23)Na(+) longitudinal magnetization and single- and triple-quantum coherences in aqueous systems containing RNA, bovine serum albumin and sodium dodecyl sulfate micelles. The effectiveness of the James-Noggle method for determining binding constants was evaluated in these systems, and also the applicability of various (23)Na coherences in providing information on the extent and affinity of binding to the three different classes of biomolecules |
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| Beschreibung: | Date Completed 11.05.2005 Date Revised 16.11.2017 published: Print Citation Status MEDLINE |
| ISSN: | 0749-1581 |