Directional genome walking using PCR
We describe here a PCR-based "directional genome walking" protocol. The basic procedure for the amplification consists of two rounds of PCR. A primary PCR was performed, on the genomic DNA using a biotinylated primer specific to a known sequence in the genome along with four universal walk...
| Publié dans: | BioTechniques. - 1991. - 33(2002), 4 vom: 14. Okt., Seite 830-2, 834 |
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| Auteur principal: | |
| Autres auteurs: | , , , |
| Format: | Article |
| Langue: | English |
| Publié: |
2002
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| Accès à la collection: | BioTechniques |
| Sujets: | Technical Report Journal Article DNA Primers DNA, Plant |