Use of CMFDA and CMTMR fluorescent dyes in FACS-based antibody screening
Cell-based immunizations are often used when membrane antigens are difficult to purify. To confirm that an antibody binding to the surface of a cell line is, in fact, binding to the desired antigen, FACS can be performed independently on two cell lines, a transfected cell line expressing the antigen...
| Veröffentlicht in: | BioTechniques. - 1993. - 32(2002), 3 vom: 28. März, Seite 678-80, 682 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2002
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Technical Report Journal Article (((4-chloromethyl)benzoyl)amino)-tetramethylrhodamine Antibodies Antigens, Neoplasm Fluoresceins Fluorescent Dyes Rhodamines 5-chloromethylfluorescein 136832-63-8 |