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231222s2001 xx ||||| 00| ||eng c |
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Yang, Y S
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Cre stoplight
|b a red/green fluorescent reporter of Cre recombinase expression in living cells
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|c 2001
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 18.04.2002
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|a Date Revised 28.09.2018
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|a published: Print
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|a Citation Status MEDLINE
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|a The Cre/lox system is a powerful genetic tool with which to manipulate the genome. Here, we describe the development of a simple reporter system for Cre recombinase, called the Cre Stoplight. In the absence of Cre, the red fluorescent protein is expressed; when Cre catalyzes a recombination event, the green fluorescent protein is produced. Testing this system in transiently transfected cells showed that it produced robust signals (90% of the cells converted from red to green) when equal amounts of the plasmids encoding Cre recombinase and the Cre Stoplight were used. A 1:100 ratio of enzyme to reporter plasmid produced similar results, and a 1:10000 ratio was necessary to significantly reduce the number of cells converting to green (1%)
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Research Support, U.S. Gov't, P.H.S.
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|a Luminescent Proteins
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|a Viral Proteins
|2 NLM
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|a fluorescent protein 583
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| 650 |
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|a Green Fluorescent Proteins
|2 NLM
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| 650 |
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|a 147336-22-9
|2 NLM
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| 650 |
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|a Cre recombinase
|2 NLM
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| 650 |
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|a EC 2.7.7.-
|2 NLM
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| 650 |
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|a Integrases
|2 NLM
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| 650 |
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|a EC 2.7.7.-
|2 NLM
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| 700 |
1 |
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|a Hughes, T E
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t BioTechniques
|d 1993
|g 31(2001), 5 vom: 16. Nov., Seite 1036, 1038, 1040-1
|w (DE-627)NLM012627046
|x 0736-6205
|7 nnns
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| 773 |
1 |
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|g volume:31
|g year:2001
|g number:5
|g day:16
|g month:11
|g pages:1036, 1038, 1040-1
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|d 31
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