Construction of recombinant vaccinia viruses using PUV-inactivated virus as a helper
Recombinant vaccinia viruses (VVs) are widely used as expression vectors in molecular biology and immunology and are now under evaluation for gene therapy. The current techniques for inserting foreign DNA into the large VV genome are based on either homologous recombination between transfer plasmids...
| Veröffentlicht in: | BioTechniques. - 1991. - 31(2001), 3 vom: 14. Sept., Seite 534, 536, 538-40 |
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| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2001
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Technical Report Journal Article DNA, Recombinant DNA, Viral Thymidine Kinase EC 2.7.1.21 Deoxyribonucleases, Type II Site-Specific EC 3.1.21.4 GCGGCCGC-specific type II deoxyribonucleases beta-Galactosidase mehr... |