Cloning full-length, cap-trapper-selected cDNAs by using the single-strand linker ligation method

Cloning full-length, cap-trapper-selected cDNAs by using the single-strand linker ligation method

We have developed the single-strand linker ligation method (SSLLM), which uses DNA ligase to add a dsDNA linker to single-stranded (ss) full-length cDNA. The linkers have random 6-bp (dN6 or dGN5) 3' overhangs that can ligate to any cDNA sequence, thereby facilitating the production of cDNA lib...

Description complète

Détails bibliographiques
Publié dans:BioTechniques. - 1993. - 30(2001), 6 vom: 02. Juni, Seite 1250-4
Auteur principal: Shibata, Y (Auteur)
Autres auteurs: Carninci, P, Watahiki, A, Shiraki, T, Konno, H, Muramatsu, M, Hayashizaki, Y
Format: Article
Langue:English
Publié: 2001
Accès à la collection:BioTechniques
Sujets:Journal Article Research Support, Non-U.S. Gov't 5' Untranslated Regions DNA, Complementary DNA, Single-Stranded Oligodeoxyribonucleotides RNA, Messenger DNA Ligases EC 6.5.1.-