Rapid selection against truncation mutants in yeast reverse two-hybrid screens
The yeast reverse two-hybrid system is a powerful technique for isolating mutations in a protein that abolish its interaction with a known partner. Selection is based on abrogation of growth suppression imposed when wild-type interactions confer 5-fluoroorotic acid (5-FOA) sensitivity to yeast cells...
| Veröffentlicht in: | BioTechniques. - 1993. - 30(2001), 5 vom: 01. Mai, Seite 984-8 |
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| Format: | Aufsatz |
| Sprache: | English |
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2001
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Technical Report Journal Article Apoptosis Regulatory Proteins Bcl-2-Like Protein 11 Carrier Proteins Drosophila Proteins Luminescent Proteins Membrane Proteins mehr... |