Pitfall of an internal control plasmid : response of Renilla luciferase (pRL-TK) plasmid to dihydrotestosterone and dexamethasone
The thymidine kinase promoter-Renilla luciferase reporter plasmid (pRL-TK) is commonly used as a control for transfection efficiency in the Dual-Luciferase Reporter Assay System. While investigating hormone response elements in the promoters of the androgen-dependent, epididymis-specific EP2 gene, w...
| Publié dans: | BioTechniques. - 1991. - 29(2000), 4 vom: 06. Okt., Seite 782-4 |
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| Auteur principal: | |
| Autres auteurs: | , , , |
| Format: | Article |
| Langue: | English |
| Publié: |
2000
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Research Support, U.S. Gov't, P.H.S. Dihydrotestosterone 08J2K08A3Y Dexamethasone 7S5I7G3JQL Luciferases EC 1.13.12.- Thymidine Kinase EC 2.7.1.21 |