Rapid colchicine competition-binding scintillation proximity assay using biotin-labeled tubulin
We have developed a rapid [3H]colchicine competition-binding scintillation proximity assay (SPA) to evaluate antimitotic compounds that bind to the colchicine-binding site on tubulin. The premise of our assay is that compounds will compete with radiolabeled colchicine for the tubulin-binding domain....
| Publié dans: | BioTechniques. - 1993. - 29(2000), 1 vom: 17. Juli, Seite 156-60 |
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| Auteur principal: | |
| Autres auteurs: | , , |
| Format: | Article |
| Langue: | English |
| Publié: |
2000
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Aminophenols Bibenzyls Solvents Stilbenes Sulfonamides Tubulin Tritium 10028-17-8 E 7010 plus... |