Vectors for expressing T7 epitope- and His6 affinity-tagged fusion proteins in S. cerevisiae
We have constructed a series of vectors (YGALSETs) for the expression of epitope- and affinity-tagged fusion proteins in yeast cells using the regulated GAL10 promoter. Fusion proteins produced from YGALSET plasmids include a leader peptide at the N terminus that encodes both a T7 gene 10 epitope ta...
Veröffentlicht in: | BioTechniques. - 1991. - 24(1998), 5 vom: 24. Mai, Seite 782-6, 788 |
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Weitere Verfasser: | , |
Format: | Aufsatz |
Sprache: | English |
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1998
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Zugriff auf das übergeordnete Werk: | BioTechniques |
Schlagworte: | Research Support, U.S. Gov't, P.H.S. Technical Report Journal Article Affinity Labels Epitopes Recombinant Fusion Proteins Histidine 4QD397987E |