High-resolution analysis of DNA replication in released chromatin fibers containing 5-bromodeoxyuridine
A strategy has been devised to physically map replication sites in released chromatin of mammalian cells. When added to the culture medium, 5-bromodeoxyuridine (BrdU) is incorporated into replicating DNA, partially replacing thymidine. BrdU pulses as short as one minute can be visualized on preparat...
| Publié dans: | BioTechniques. - 1993. - 21(1996), 6 vom: 15. Dez., Seite 1050-4 |
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| Auteur principal: | |
| Format: | Article |
| Langue: | English |
| Publié: |
1996
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| Accès à la collection: | BioTechniques |
| Sujets: | Technical Report Journal Article Chromatin DNA 9007-49-2 Bromodeoxyuridine G34N38R2N1 |