Detection of calmodulin-binding proteins using a 32P-labeled GST-calmodulin fusion protein and a novel renaturation protocol
To identify calmodulin-binding proteins in cellular extracts and tissue homogenates and to analyze purified calmodulin target proteins, overlay procedures using 125I-calmodulin or, more recently, nonradioactive biotinylated calmodulin have been widely used. Here we describe a rapid, alternative meth...
| Publié dans: | BioTechniques. - 1991. - 21(1996), 2 vom: 23. Aug., Seite 292-6 |
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| Auteur principal: | |
| Autres auteurs: | , |
| Format: | Article |
| Langue: | English |
| Publié: |
1996
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't Calmodulin Calmodulin-Binding Proteins Parvalbumins Phosphorus Radioisotopes Recombinant Fusion Proteins Glutathione Transferase EC 2.5.1.18 Cyclic AMP-Dependent Protein Kinases plus... |