Rapid bright-field detection of oligonucleotide primed in situ (PRINS)-labeled DNA in chromosome preparations and frozen tissue sections

We describe a new application of a bright-field microscopic procedure for rapid enzyme cytochemical detection of repeated DNA sequences in metaphase preparations and frozen tissue sections. Various chromosome-specific oligonucleotide primers were used in up to three sequential primed in situ (PRINS)...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1991. - 20(1996), 2 vom: 29. Feb., Seite 226-34
1. Verfasser: Speel, E J (VerfasserIn)
Weitere Verfasser: Lawson, D, Ramaekers, F C, Gosden, J R, Hopman, A H
Format: Aufsatz
Sprache:English
Veröffentlicht: 1996
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't DNA Primers Deoxyribonucleotides Fluorescent Dyes Biotin 6SO6U10H04 DNA 9007-49-2 Horseradish Peroxidase mehr... EC 1.11.1.- DNA-Directed DNA Polymerase EC 2.7.7.7 Digoxigenin NQ1SX9LNAU