Improved method for the production of M13 phage and single-stranded DNA for DNA sequencing
An improved method is described for the efficient production of M13 phage and M13 single-stranded (ss)DNA in a relatively short time period. Infection of E. coli (F') cells with as few as 5 phage particles can yield 10(12) phage particles/mL in 3 hours if the cells are grown in LB broth or SOB...
| Publié dans: | BioTechniques. - 1991. - 20(1996), 5 vom: 01. Mai, Seite 854-6, 858, 860 |
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| Format: | Article |
| Langue: | English |
| Publié: |
1996
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Culture Media DNA, Single-Stranded DNA, Viral |