Optimization of competitor poly(dI-dC).poly(dI-dC) levels is advised in DNA-protein interaction studies involving enriched nuclear proteins

Optimization of competitor poly(dI-dC).poly(dI-dC) levels is advised in DNA-protein interaction studies involving enriched nuclear proteins

Procedures used for investigating DNA-protein interactions, such as the electrophoretic mobility shift assay (EMSA) or DNasel footprinting, require that exogenous nucleic acids (or synthetic equivalents) be added to the reaction mixture to prevent or reduce the nonspecific interaction of nuclear pro...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1993. - 20(1996), 3 vom: 22. März, Seite 439-44
1. Verfasser: Larouche, K (VerfasserIn)
Weitere Verfasser: Bergeron, M J, Leclerc, S, Guérin, S L
Format: Aufsatz
Sprache:English
Veröffentlicht: 1996
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Research Support, Non-U.S. Gov't Technical Report Journal Article Nuclear Proteins Polydeoxyribonucleotides Sp1 Transcription Factor poly d(I-C) 25853-45-6 DNA 9007-49-2