A PCR-based strategy for extensive mutagenesis of a target DNA sequence
A mixed population of mutagenic oligonucleotide primers was used to generate a set of point mutations in a short region of a retroviral gene by PCR amplification. The mixed population of mutagenic primers was generated by incorporating a mixture of A, G, C and T at specific sites during oligonucleot...
| Publié dans: | BioTechniques. - 1991. - 14(1993), 3 vom: 04. März, Seite 454-7 |
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| Format: | Article |
| Langue: | English |
| Publié: |
1993
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Research Support, U.S. Gov't, P.H.S. DNA, Viral Gene Products, env Retroviridae Proteins, Oncogenic Viral Fusion Proteins transmembrane protein, Simian immunodeficiency virus DNA 9007-49-2 |