A PCR-based method for site-specific domain replacement that does not require restriction recognition sequences
We describe a PCR-based method for domain replacement that does not require restriction site sequences. We illustrate the technique in which the first epidermal growth factor (EGF1)-like domain of factor IX (FIX) is replaced by the EGF1-like domain of protein C. The method employs four oligonucleoti...
| Veröffentlicht in: | BioTechniques. - 1993. - 15(1993), 5 vom: 15. Nov., Seite 874-8 |
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| Format: | Aufsatz |
| Sprache: | English |
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1993
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Technical Report Journal Article DNA Primers Protein C Recombinant Fusion Proteins Epidermal Growth Factor 62229-50-9 Factor IX mehr... |