Profile matching and profile subtraction : application of computer-based image analysis system for two-dimensional electrophoresis gels

The microcomputer-based image analysis system IB-1000 (developed by Indiana Biotech, Highland, IN) for two-dimensional electrophoresis gels has been described previously (9). It allows the user to compare protein spots between two profiles and identify those spots that are commonly shared in both pr...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1991. - 7(1989), 4 vom: 08. Apr., Seite 374-8
1. Verfasser: Lee, C (VerfasserIn)
Weitere Verfasser: Sherwood, E R, Sensibar, J A, Berg, L A, Chen, Y C, Tseng, C C
Format: Aufsatz
Sprache:English
Veröffentlicht: 1989
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, U.S. Gov't, P.H.S. Proteins
Beschreibung
Zusammenfassung:The microcomputer-based image analysis system IB-1000 (developed by Indiana Biotech, Highland, IN) for two-dimensional electrophoresis gels has been described previously (9). It allows the user to compare protein spots between two profiles and identify those spots that are commonly shared in both profiles. This report describes two applications of the system's global comparison routine-profile matching and profile subtraction. This application is able to subtract commonly shared spots from one profile, creating a new profile made up by the unmatched spots in the other profile. These applications can be employed in a large variety of research projects
Beschreibung:Date Completed 01.05.1990
Date Revised 30.11.2018
published: Print
Citation Status MEDLINE
ISSN:1940-9818