Solid-phase direct DNA sequencing of allele-specific polymerase chain reaction-amplified HLA-DR genes
We describe in this report a new strategy to directly sequence polymerase chain reaction-amplified human leucocyte antigen DRB genes using biotinylated allele-specific synthetic oligonucleotide primers coupled to streptavidin-coated magnetic beads. The use of allele-specific primers in the polymeras...
| Publié dans: | BioTechniques. - 1991. - 10(1991), 1 vom: 05. Jan., Seite 30, 32, 34 |
|---|---|
| Auteur principal: | |
| Autres auteurs: | , , , |
| Format: | Article |
| Langue: | English |
| Publié: |
1991
|
| Accès à la collection: | BioTechniques |
| Sujets: | Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Technical Report Journal Article HLA-DR Antigens Oligodeoxyribonucleotides |