Comparison and modification of rRNA sequencing methods
In this study, modification of two methods of RNA sequencing resulted in more definitive sequencing bands. In one method of sequencing, the bands of lane A and lane G sometimes were not clear. Modifications of this method by changing the concentrations of ddATP and ddGTP resulted in the bands of lan...
| Publié dans: | BioTechniques. - 1991. - 11(1991), 4 vom: 01. Okt., Seite 438, 440 |
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| Auteur principal: | |
| Autres auteurs: | , |
| Format: | Article |
| Langue: | English |
| Publié: |
1991
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| Accès à la collection: | BioTechniques |
| Sujets: | Comparative Study Journal Article Research Support, Non-U.S. Gov't RNA, Ribosomal RNA, Ribosomal, 16S DNA 9007-49-2 |
| Résumé: | In this study, modification of two methods of RNA sequencing resulted in more definitive sequencing bands. In one method of sequencing, the bands of lane A and lane G sometimes were not clear. Modifications of this method by changing the concentrations of ddATP and ddGTP resulted in the bands of lane A and lane G becoming more readable. Although a second sequencing method was found to have clearer bands than the first method, and the bases immediately downstream from the primer binding site could be read by using r-32P-labeled primer, the bands on the top of lane A still were not clear. Modifications of this second method by changing the ddATP/dATP ratio resulted in the bands of lane A becoming much clearer |
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| Description: | Date Completed 08.04.1992 Date Revised 15.11.2006 published: Print Citation Status MEDLINE |
| ISSN: | 1940-9818 |