PCR amplification and simultaneous digoxigenin incorporation of long DNA probes for fluorescence in situ hybridization
Nonradioactive in situ hybridization has found widespread applications in cytogenetics. Basic requirements are DNA probes in sufficient amounts and of high specificity as well as a labeling protocol of good reproducibility. The PCR has been of fundamental importance for the amplification of DNA sequ...
| Publié dans: | BioTechniques. - 1988. - 12(1992), 1 vom: 09. Jan., Seite 98-102 |
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| Auteur principal: | |
| Autres auteurs: | , |
| Format: | Article |
| Langue: | English |
| Publié: |
1992
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't DNA Probes Deoxyuracil Nucleotides Fluorescent Dyes Indicators and Reagents digoxigenin-11-deoxyuridine triphosphate Fluorescein-5-isothiocyanate I223NX31W9 Digoxigenin |