RNA template-specific PCR an improved method that dramatically reduces false positives in RT-PCR

RNA template-specific PCR : an improved method that dramatically reduces false positives in RT-PCR

We report a novel modification of the reverse transcription PCR method, designated RNA template-specific PCR. With this approach, the 5' end of the first strand is tagged with a unique nucleotide sequence during reverse transcription that may then be exploited to amplify preferentially RNA-deri...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1991. - 11(1991), 6 vom: 26. Dez., Seite 760-3
1. Verfasser: Shuldiner, A R (VerfasserIn)
Weitere Verfasser: Tanner, K, Moore, C A, Roth, J
Format: Aufsatz
Sprache:English
Veröffentlicht: 1991
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Insulin RNA 63231-63-0 DNA 9007-49-2 RNA-Directed DNA Polymerase EC 2.7.7.49