Full-length cDNA cloning utilizing the polymerase chain reaction, a degenerate oligonucleotide sequence and a universal mRNA primer

A full-length cDNA was selectively amplified by the polymerase chain reaction (PCR) utilizing a primer pair consisting of a "universal" 21-base synthetic deoxyoligonucleotide (oligo dT 17GGCC) and a specific degenerate deoxyoligonucleotide sequence (DOS) derived from the N-terminal amino a...

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Détails bibliographiques
Publié dans:BioTechniques. - 1991. - 9(1990), 1 vom: 31. Juli, Seite 60-5
Auteur principal: Cooper, D L (Auteur)
Autres auteurs: Isola, N
Format: Article
Langue:English
Publié: 1990
Accès à la collection:BioTechniques
Sujets:Journal Article Oligodeoxyribonucleotides RNA primers RNA, Messenger RNA, Neoplasm Poly A 24937-83-5 RNA 63231-63-0 DNA 9007-49-2