Simplified northern blot hybridization using 5% sodium dodecyl sulfate

Simplified northern blot hybridization using 5% sodium dodecyl sulfate

We describe a simplified Northern blot hybridization procedure. This procedure eliminates the need for many reagents commonly used in RNA hybridization and replaces them with two buffers containing 5% sodium dodecyl sulfate for pre-hybridization, hybridization and all post-hybridization washes

Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1991. - 8(1990), 4 vom: 01. Apr., Seite 370-1
1. Verfasser: Virca, G D (VerfasserIn)
Weitere Verfasser: Northemann, W, Shiels, B R, Widera, G, Broome, S
Format: Aufsatz
Sprache:English
Veröffentlicht: 1990
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article DNA Probes Sodium Dodecyl Sulfate 368GB5141J RNA 63231-63-0
Beschreibung
Zusammenfassung:We describe a simplified Northern blot hybridization procedure. This procedure eliminates the need for many reagents commonly used in RNA hybridization and replaces them with two buffers containing 5% sodium dodecyl sulfate for pre-hybridization, hybridization and all post-hybridization washes
Beschreibung:Date Completed 25.06.1990
Date Revised 21.11.2013
published: Print
Citation Status MEDLINE
ISSN:1940-9818