High-efficiency cDNA cloning : a comparison of electroporation and in vitro packaging
The production of complete cDNA libraries from minimal amounts of starting material (i.e., mRNA) is a major challenge for cDNA cloning technology. This paper reports the cDNA cloning efficiencies of electroporation and in vitro packaging. These two methods produce a tremendous number of recombinant...
| Veröffentlicht in: | BioTechniques. - 1991. - 12(1992), 6 vom: 08. Juni, Seite 804-6, 808 |
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| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1992
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Comparative Study Journal Article |
| Zusammenfassung: | The production of complete cDNA libraries from minimal amounts of starting material (i.e., mRNA) is a major challenge for cDNA cloning technology. This paper reports the cDNA cloning efficiencies of electroporation and in vitro packaging. These two methods produce a tremendous number of recombinant clones (greater than or equal to 1 x 10(8) clones/micrograms cDNA) although electroporation generates more clones at nearly all cDNA concentrations used in a ligation reaction |
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| Beschreibung: | Date Completed 10.09.1992 Date Revised 15.11.2006 published: Print Citation Status MEDLINE |
| ISSN: | 1940-9818 |