Use of a cellular ELISA for the detection of cell surface antigens
A sensitive, convenient and inexpensive enzyme-linked immunosorbent assay (ELISA) is described for the detection and relative quantitation of cell surface antigens. The cells to be tested are rapidly glutaraldehyde-fixed to the wells of microtiter plates, which can be stored for later assay, if desi...
| Publié dans: | BioTechniques. - 1991. - 12(1992), 3 vom: 15. März, Seite 326-30 |
|---|---|
| Auteur principal: | |
| Autres auteurs: | |
| Format: | Article |
| Langue: | English |
| Publié: |
1992
|
| Accès à la collection: | BioTechniques |
| Sujets: | Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Antigens, Surface |
| Résumé: | A sensitive, convenient and inexpensive enzyme-linked immunosorbent assay (ELISA) is described for the detection and relative quantitation of cell surface antigens. The cells to be tested are rapidly glutaraldehyde-fixed to the wells of microtiter plates, which can be stored for later assay, if desired. Alternatively, adherent cells may be left unfixed. Following incubation with antibodies specific for the antigens of interest, an enzyme-linked second antibody conjugate is added, followed by the substrate for the enzyme, as in a conventional ELISA for soluble proteins. The method is a sensitive and accurate alternative to immunofluorescence flow cytometry for rapid and inexpensive screening of large numbers of cell samples |
|---|---|
| Description: | Date Completed 29.05.1992 Date Revised 15.11.2007 published: Print Citation Status MEDLINE |
| ISSN: | 1940-9818 |