Recombinant circle PCR and recombination PCR for site-specific mutagenesis without PCR product purification
Two simple methods for site-specific mutagenesis are described and compared. In each method, the PCR is used in two separate amplifications to mutate the site of interest and to add ends to one PCR product that are homologous to the ends of the other PCR product. In the first method, the two product...
| Publié dans: | BioTechniques. - 1991. - 12(1992), 4 vom: 15. Apr., Seite 528-30, 532, 534-5 |
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| Format: | Article |
| Langue: | English |
| Publié: |
1992
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| Accès à la collection: | BioTechniques |
| Sujets: | Comparative Study Journal Article Research Support, Non-U.S. Gov't DNA Probes |