CircumVent thermal cycle sequencing and alternative manual and automated DNA sequencing protocols using the highly thermostable VentR (exo-) DNA polymerase

CircumVent thermal cycle and standard DNA sequencing protocols utilizing the cloned and highly thermostable VentR (exo-) DNA polymerase are described. The thermal cycle sequencing procedures are advantageous because they allow fast and simple semiautomation of the sequencing reaction; make possible...

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Détails bibliographiques
Publié dans:BioTechniques. - 1988. - 13(1992), 4 vom: 12. Okt., Seite 626-33
Auteur principal: Sears, L E (Auteur)
Autres auteurs: Moran, L S, Kissinger, C, Creasey, T, Perry-O'Keefe, H, Roskey, M, Sutherland, E, Slatko, B E
Format: Article
Langue:English
Publié: 1992
Accès à la collection:BioTechniques
Sujets:Journal Article Research Support, Non-U.S. Gov't DNA 9007-49-2 Tli polymerase EC 2.7.7.- DNA-Directed DNA Polymerase EC 2.7.7.7