A nonradioactive micro-assay for released reverse transcriptase activity of a lentivirus
A nonradioactive micro-assay procedure for detection of released reverse transcriptase activity from cells infected with equine infectious anemia virus is described. This procedure utilizes biotinylated-dUTP in conjunction with a streptavidin-alkaline phosphatase conjugate. Detection of alkaline pho...
| Publié dans: | BioTechniques. - 1991. - 13(1992), 3 vom: 10. Sept., Seite 380-6 |
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| Auteur principal: | |
| Autres auteurs: | , |
| Format: | Article |
| Langue: | English |
| Publié: |
1992
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Bacterial Proteins Biotin 6SO6U10H04 Streptavidin 9013-20-1 RNA-Directed DNA Polymerase EC 2.7.7.49 plus... |
| Résumé: | A nonradioactive micro-assay procedure for detection of released reverse transcriptase activity from cells infected with equine infectious anemia virus is described. This procedure utilizes biotinylated-dUTP in conjunction with a streptavidin-alkaline phosphatase conjugate. Detection of alkaline phosphatase is by autoradiography of the chemiluminescence produced during enzymatic dephosphorylation of Lumi Phos 530. This method, as with reverse transcriptase micro-assays employing 32P-labeled nucleotides, is suited to the processing of numerous samples, while having the advantages of safety and stability normally associated with nonradioactive methods of detection. Sensitivity is comparable to a reverse transcriptase micro-assay using 32P-dTTP |
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| Description: | Date Completed 19.11.1992 Date Revised 21.11.2013 published: Print Citation Status MEDLINE |
| ISSN: | 1940-9818 |