Preparation of plasmid DNA by sequential enzymatic digestion
A new method for the preparation of plasmid DNA from Escherichia coli, sequential enzymatic digestion, is described. The method is based on sequential and selective enzymatic digestion of all components of E. coli except for the supercoiled plasmid DNA. The key enzymes are exonuclease I and exonucle...
| Publié dans: | BioTechniques. - 1991. - 13(1992), 4 vom: 08. Okt., Seite 550-4 |
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| Auteur principal: | |
| Format: | Article |
| Langue: | English |
| Publié: |
1992
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article DNA, Bacterial Enzymes Exodeoxyribonucleases EC 3.1.- exodeoxyribonuclease I EC 3.1.11.1 exodeoxyribonuclease III EC 3.1.11.2 |