Gene transfer into established and primary fibroblast cell lines : comparison of transfection methods and promoters

The stable transfection of immortalized Rat-1 and rat skin primary fibroblast cell lines by calcium phosphate precipitation, lipofection and electroporation methods have been examined. The lipofection method was found to be better than the other methods in terms of higher transfection efficiency and...

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Publié dans:BioTechniques. - 1988. - 13(1992), 4 vom: 30. Okt., Seite 598-603
Auteur principal: Ray, J (Auteur)
Autres auteurs: Gage, F H
Format: Article
Langue:English
Publié: 1992
Accès à la collection:BioTechniques
Sujets:Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Calcium Phosphates alpha-tricalcium phosphate tetracalcium phosphate calcium phosphate, monobasic, anhydrous 701EKV9RMN calcium phosphate plus... 97Z1WI3NDX beta-Galactosidase EC 3.2.1.23 Neomycin I16QD7X297 calcium phosphate, dibasic, anhydrous L11K75P92J